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B95 8 cell line.
Thus it provides a source of ebv to establish continuous lymphocytic lines from human donors.
The b95 8 cell line s name originates from the b95 strain of epstein barr virus ebv.
The viral strain was isolatedfrom a case of transfusion induced mononucleosis and subsequently used in vitro to immortalise monkey blood leukocytes miller et al pnas 1972 to generate the cell line.
Marmoset used to be described in the animal name section was a mistake and corrected to the name cotton top tamarin with the scientific name.
The b95 8 cell line must be handled in the p2 labotory since ebv can infect primates.
Releases high titres of transforming ebv.
The ebv producing marmoset b cell line b95 8 commonly used as a source of ebv for stimulation and transformation of human b cells was shown to proliferate in response to supernatants containing human b cell growth factors bcgf derived from pha activated t cells or the kg la cell line and to a commercial low molecular weight bcgf bcgflow but not to recombinant human il 4.
Marmoset used to be described in the animal name section was a mistake and corrected to the name cotton top tamarin with the scientific name.
This cell line has been assigned by the zkbs german central commission for biological safety to risk group 2 ief analysis with ldh and np confirmed the cells as non.
The b95 8 cell line must be handled in the bsl 2 labotory since ebv can infect primates.
In some instances the cell line b95 8 has been described as.
B95 8 b95 8 b958 cotton top tamarin monkey peripheral blood lymphocyte derived from a cotton top tamarin saguinus oedipus.
Many investigators look for b95 8 cells atcc crl 1612 because this monkey cell line sheds the epstein barr virus that is used in transforming b lymphocytes.
Zero bias scores article reviews protocol conditions and more.
In some instances the cell line b95 8 has been described as derived from marmoset however this is not correct.
This cell line is used as a source of ebv.
B95 8 was derived from a cotton top tamarin saguinas oedipus.
This has been confirmed by dna profiling.
B95 8 cell line flask presentation1 flask 75 cm2 ftb95 information request.
Through the use of dna profiling it has now been shown.
94 100 based on 2 pubmed citations.
The cells should be handled under laboratory containment level 2.
Ebv providing a source for transformation of b cells and establishment of continuous cell lines.
However the cell line is derived from a primate species that is listed as endangered by the u s.
B95 8 marmoset leukocyte cell line supplied by atcc used in various techniques.
B95 8 monkey marmoset lymphocyte b peripheral species.
